Protocol Link Library

General Plant Tissue Culture Protocols

Covers the standard workflow: initiation, multiplication, rooting, and acclimatization12.

Includes sterilization, explant selection, and basic media preparation.

Callus Culture Protocols

Procedures for inducing and maintaining undifferentiated cell masses (callus) from various plant tissues345.

Organ Culture Protocols

Methods for culturing specific plant organs (e.g., roots, stems, leaves, flowers) to retain structure and function3.

Meristem and Bud Culture Protocols

Focuses on culturing shoot tips, apical meristems, or buds—crucial for virus elimination and clonal propagation45.

Protoplast Culture Protocols

Techniques for isolating and culturing plant cells without cell walls, often used for hybridization and genetic studies36.

Anther and Pollen Culture Protocols

Methods for producing haploid plants, commonly used in breeding and research45.

Somatic Embryogenesis Protocols

Steps for inducing embryos from somatic (non-reproductive) tissues, enabling large-scale propagation of ornamentals76.

Cell Suspension Culture Protocols

Protocols for growing plant cells in liquid media, useful for metabolite production and research45.

Media Preparation & Sterilization

Detailed recipes and sterilization techniques for preparing nutrient-rich agar or liquid media85.

Acclimatization and Transfer Protocols

Guidelines for transitioning tissue-cultured plantlets from in vitro to greenhouse or soil conditions12.
Agrobacterium Mediation
Individual Cultivar Protocols

Epipremnum aureum:

1. FACILITATION OF THE RHIZOGENESIS AND CAULOGENESIS OF EPIPREMNUM AUREUM BY IN VITRO MICROPROPAGATION AND DIFFERENT MURASHIGESKOOG CULTURE MEDIA FORMULATION

2. DEVELOPING IN VITRO PROPAGATION AND SELECTION SYSTEMS FOR AGLAONEMA AND POTHOS

Philodendron hederaceum ‘Micans’

1. REGENERATION OF PHILODENDRON MICANS K. KOCH THROUGH PROTOCORM-LIKE BODIES AND IMPROVEMENT OF PLANT FORM USING GROWTH REGULATORS

Chlorophytum comosum 'Reverse Variegatum'

1. Cultivating Spider Plants: Conventional and Tissue Culture Technique

Monstera Deliciosa

1. Monstera Deliciosa Tissue Culture Multiplication Media Protocol